A fluorescence anisotropy method for measuring protein concentration in complex cell culture media
Groza, RC,Calvet, A,Ryder, AG Groza, Radu Constantin Calvet, Amandine Ryder, Alan G.
Groza, RC,Calvet, A,Ryder, AG
Groza, Radu Constantin
Calvet, Amandine
Ryder, Alan G.
Repository DOI
Publication Date
2014-03-12
Type
Article
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Citation
A Fluorescence Anisotropy Method for Protein Concentration Monitoring in Complex Cell Culture Media. R.C. Groza, A. Calvet, and A.G. Ryder. Analytica Chimica Acta, 821, 54-61, (2014).
Abstract
The rapid, quantitative analysis of the complex cell culture media used in biopharmaceutical manufacturing is of critical importance. Requirements for cell culture media composition profiling, or changes in specific analyte concentrations (e. g. amino acids in the media or product protein in the bioprocess broth) often necessitate the use of complicated analytical methods and extensive sample handling. Rapid spectroscopic methods like multi-dimensional fluorescence (MDF) spectroscopy have been successfully applied for the routine determination of compositional changes in cell culture media and bioprocess broths. Quantifying macromolecules in cell culture media is a specific challenge as there is a need to implement measurements rapidly on the prepared media. However, the use of standard fluorescence spectroscopy is complicated by the emission overlap from many media components. Here, we demonstrate how combining anisotropy measurements with standard total synchronous fluorescence spectroscopy (TSFS) provides a rapid, accurate quantitation method for cell culture media. Anisotropy provides emission resolution between large and small fluorophores while TSFS provides a robust measurement space. Model cell culture mediawas prepared using yeastolate (2.5mgmL(-1)) spiked with bovine serum albumin (0 to 5mgmL (-1)). Using this method, protein emission is clearly discriminated from background yeastolate emission, allowing for accurate bovine serum albumin (BSA) quantification over a 0.1 to 4.0mgmL(-1)range with a limit of detection (LOD) of 13.8mgmL(-1). (c) 2014 Published by Elsevier B.V.
Funder
Publisher
Elsevier
Publisher DOI
10.1016/j.aca.2014.03.007
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Attribution-NonCommercial-NoDerivs 3.0 Ireland