Structure elucidation and in vitro toxicity of new azaspiracids isolated from the marine dinoflagellate azadinium poporum
Krock, Bernd Tillmann, Urban Potvin, Éric Jeong, Hae Drebing, Wolfgang Kilcoyne, Jane Al-Jorani, Ahmed Twiner, Michael Göthel, Qun Köck, Matthias
Krock, Bernd
Tillmann, Urban
Potvin, Éric
Jeong, Hae
Drebing, Wolfgang
Kilcoyne, Jane
Al-Jorani, Ahmed
Twiner, Michael
Göthel, Qun
Köck, Matthias
Repository DOI
Publication Date
2015-10-30
Type
Article
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Citation
Krock, Bernd; Tillmann, Urban; Potvin, Éric; Jeong, Hae; Drebing, Wolfgang; Kilcoyne, Jane; Al-Jorani, Ahmed; Twiner, Michael; Göthel, Qun; Köck, Matthias (2015). Structure elucidation and in vitro toxicity of new azaspiracids isolated from the marine dinoflagellate azadinium poporum. Marine Drugs 13 (11), 6687-6702
Abstract
Two strains of Azadinium poporum, one from the Korean West coast and the other from the North Sea, were mass cultured for isolation of new azaspiracids. Approximately 0.9 mg of pure AZA-36 (1) and 1.3 mg of pure AZA-37 (2) were isolated from the Korean (870 L) and North Sea (120 L) strains, respectively. The structures were determined to be 3-hydroxy-8-methyl-39-demethyl-azaspiracid-1 (1) and 3-hydroxy-7,8-dihydro-39-demethyl-azaspiracid-1 (2) by H-1- and C-13-NMR. Using the Jurkat T lymphocyte cell toxicity assay, (1) and (2) were found to be 6- and 3-fold less toxic than AZA-1, respectively.
Funder
Publisher
MDPI AG
Publisher DOI
10.3390/md13116687
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland