High-resolution, wide-field object reconstruction with synthetic aperture Fourier holographic optical microscopy
Hillman, Timothy R. Gutzler, Thomas Alexandrov, Sergey Sampson, David
Hillman, Timothy R.
Gutzler, Thomas
Alexandrov, Sergey
Sampson, David
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Publication Date
2009
Type
Article
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Citation
Hillman, Timothy R., Gutzler, Thomas, Alexandrov, Sergey A., & Sampson, David D. (2009). High-resolution, wide-field object reconstruction with synthetic aperture Fourier holographic optical microscopy. Optics Express, 17(10), 7873-7892. doi: 10.1364/OE.17.007873
Abstract
We utilize synthetic-aperture Fourier holographic microscopy to resolve micrometer-scale microstructure over millimeter-scale fields of view. Multiple holograms are recorded, each registering a different, limited region of the sample object’s Fourier spectrum. They are “stitched together” to generate the synthetic aperture. A low-numerical-aperture (NA) objective lens provides the wide field of view, and the additional advantages of a long working distance, no immersion fluids, and an inexpensive, simple optical system. Following the first theoretical treatment of the technique, we present images of a microchip target derived from an annular synthetic aperture (NA = 0.61) whose area is 15 times that due to a single hologram (NA = 0.13); they exhibit a corresponding qualitative improvement. We demonstrate that a high-quality reconstruction may be obtained from a limited sub-region of Fourier space, if the object’s structural information is concentrated there.
Funder
Publisher
Optica
Publisher DOI
10.1364/OE.17.007873
Rights
CC BY-NC-ND 3.0 IE