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Cloning and characterization of the rainbow trout (oncorhynchus mykiss) type ii interleukin-1 receptor cdna

Sangrador-Vegas, Amaia
; 
Martin, Samuel A. M.
; 
O’Dea, Paul G.
; 
Smith, Terry J.
Repository DOI
Publication Date
2000-12-01
Keywords
cdna sequence, differential hybridization, oncorhynchus mykiss, suppression subtractive hybridization, type ii interleukin-1 receptor, suppression subtractive hybridization, il-1 receptor, molecular-cloning, messenger-rna, decoy target, expression, chemokine, affinity, gene, identification
Type
Article
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Citation
Sangrador-Vegas, Amaia; Martin, Samuel A. M. O’Dea, Paul G.; Smith, Terry J. (2000). Cloning and characterization of the rainbow trout (oncorhynchus mykiss) type ii interleukin-1 receptor cdna. European Journal of Biochemistry 267 (24), 7031-7037
Abstract
A homologue of mammalian type II interleukin-1 receptor (IL-1RII) was isolated from a rainbow trout cDNA library by differential hybridization using a suppression subtractive hybridization generated probe enriched far sequences upregulated after immune stimulation. The trout cDNA has an ORF encoding 441 amino acids, and represents the first piscine IL-1 receptor described. The predicted amino-acid sequence has 29 and 26% identity with human and mouse IL-1RII, respectively. The trout IL-1 receptor has a domain organization similar to that of mammalian type II receptor, with a short cytoplasmic tail of 24 amino acids. These results suggest that type II receptor is also present in lower vertebrates, and therefore the duplication of an ancestral gene that generated type I and type II IL-1 receptors occurred prior to the time mammals emerged.
Funder
Publisher
Wiley-Blackwell
Publisher DOI
10.1046/j.1432-1327.2000.01800.x
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (1)