Publication

Dna-pk activity is associated with caspase-dependent myogenic differentiation

Connolly, Patrick F.
; 
Fearnhead, Howard O.
Repository DOI
Publication Date
2016-10-01
Keywords
apoptosis, caspase-3, differentiation, DNA-damage, DNA-pk, muscle, myogenesis, kinase catalytic subunit, protein-kinase, muscle differentiation, nonsense mutation, damage, gene, h2ax, identification, replication, repair
Type
Article
Downloads
Show all metadata
Citation
Connolly, Patrick F. Fearnhead, Howard O. (2016). Dna-pk activity is associated with caspase-dependent myogenic differentiation. The FEBS Journal 283 (19), 3626-3636
Abstract
Differentiation of myoblasts into myotubes is essential for skeletal muscle development and regeneration. Caspase-3 and caspase-9 are required for efficient myoblast differentiation. The caspase-activated endonuclease activity, CAD, and the DNA-damage repair protein XRCC1 have also been shown to be required to complete differentiation. DNA-damage associated with differentiation is accompanied by phosphorylation of Histone 2AX, an event normally catalysed by kinases ATR, ATM or DNA-PK. However, the kinase responsible for phosphorylation during differentiation is not known. Here we show that inhibition of DNA-PK, but not of ATR or ATM, blocked histone phosphorylation during differentiation. We also show that DNA-PK inhibition and siRNA-mediated DNA-PK knockdown blocked cell fusion. These data implicate a new role for DNA-PK in myogenic differentiation.
Funder
Publisher
Wiley-Blackwell
Publisher DOI
10.1111/febs.13832
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
cbn
Collections
Externally hosted open access publications with University of Galway authors (2)