Comparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzae
Coughlan, Helena Reddington, Kate Tuite, Nina Wee Boo, Teck Cormican, Martin Barrett, Louise Smith, Terry Clancy, Eoin Barry, Thomas
Coughlan, Helena
Reddington, Kate
Tuite, Nina
Wee Boo, Teck
Cormican, Martin
Barrett, Louise
Smith, Terry
Clancy, Eoin
Barry, Thomas
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Publication Date
2015-06-23
Type
Article
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Citation
Helena Coughlan, Kate Reddington, Nina Tuite, Teck Wee Boo, Martin Cormican, Louise Barrett, Terry J Smith, Eoin Clancy, Thomas Barry (2015) 'Comparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzae'. Diagnostic Microbiology And Infectious Disease, 83 (2):112-116.
Abstract
Haemophilus influenzae is recognised as an important human pathogen associated with invasive infections, including bloodstream infection and meningitis. Currently used molecular-based diagnostic assays lack specificity in correctly detecting and identifying H. influenzae. As such, there is a need to develop novel diagnostic assays for the specific identification of H. influenzae. Whole genome comparative analysis was performed to identify putative diagnostic targets, which are unique in nucleotide sequence to H. influenzae. From this analysis, we identified 2 H. influenzae putative diagnostic targets, phoB and pstA, for use in real-time PCR diagnostic assays. Real-time PCR diagnostic assays using these targets were designed and optimised to specifically detect and identify all 55 H. influenzae strains tested. These novel rapid assays can be applied to the specific detection and identification of H. influenzae for use in epidemiological studies and could also enable improved monitoring of invasive disease caused by these bacteria.
Funder
Publisher
Elsevier
Publisher DOI
10.1016/j.diagmicrobio.2015.06.013
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Attribution-NonCommercial-NoDerivs 3.0 Ireland