Experimental and computational examination of anastellin (FnIII1c)-polymer interactions
Mallinson, David Cheung, David L. Simionesie, Dorin Mullen, Alexander B. Zhang, Zhenyu J. Lamprou, Dimitrios A.
Mallinson, David
Cheung, David L.
Simionesie, Dorin
Mullen, Alexander B.
Zhang, Zhenyu J.
Lamprou, Dimitrios A.
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Publication Date
2016-10-24
Type
Article
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Mallinson, D., Cheung, D. L., Simionesie, D., Mullen, A. B., Zhang, Z. J. and Lamprou, D. A. (2016), Experimental and computational examination of anastellin (FnIII1c)-polymer interactions. J. Biomed. Mater. Res.. Accepted Author Manuscript. doi:10.1002/jbm.a.35949
Abstract
Using a combination of experimental and computational approaches, the interaction between anastellin, a recombinant fragment of fibronectin, and representative biomaterial surfaces has been examined. Anastellin and superfibronectin, have been seen to exhibit anti-angiogenic properties and other properties that may make it suitable for consideration for incorporation into biomaterials. The molecular interaction was directly quantified by atomic force microscope (AFM) based force spectroscopy, complemented by adsorption measurements using quartz crystal microbalance (QCM). By AFM, it was found that the anastellin molecule facilitates a stronger adhesion on polyurethane films (72.0 pN nm-1 ) than on poly (methyl methacrylate) films (68.6 pN nm-1 ). However, this is not consistent with the QCM adsorption measurements which shows no significant difference. Molecular dynamics simulations of the behaviour of anastellin on polyurethane in aqueous solution were performed to rationalise the experimental data, and show that anastellin is capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in water.
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Publisher
Wiley
Publisher DOI
10.1002/jbm.a.35949
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Attribution-NonCommercial-NoDerivs 3.0 Ireland