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Chemical structures of the core regions of campylobacter jejuni serotypes o:1, o:4, o:23, and o:36 lipopolysaccharides

ASPINALL, Gerald O.
; 
McDONALD, Armando G.
; 
RAJU, T. Shantha
; 
PANG, Henrianna
; 
MORAN, Anthony P.
; 
PENNER, John L.
Repository DOI
Publication Date
1993-05-01
Keywords
bombardment mass-spectrometry, neisseria-gonorrhoeae, oligosaccharide epitopes, monoclonal-antibodies, lipooligosaccharides, meningitidis, polysaccharide, sialylation, resistance, expression
Type
Article
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Citation
ASPINALL, Gerald O. McDONALD, Armando G.; RAJU, T. Shantha; PANG, Henrianna; MORAN, Anthony P.; PENNER, John L. (1993). Chemical structures of the core regions of campylobacter jejuni serotypes o:1, o:4, o:23, and o:36 lipopolysaccharides. European Journal of Biochemistry 213 (3), 1017-1027
Abstract
Complete structures, including the location of N-acetylneuraminic acid (Neu5Ac) residues, were assigned for the core regions of Campylobacter jejuni serotypes 0:1, 0:4, and 0:23 and 0:36 lipopolysaccharides (LPS). In continuation of earlier studies, structure determinations of liberated oligosaccharides and, where necessary, of intact LPS, were by H-1-NMR spectroscopy, Smith degradation, chromium trioxide and enzymic degradations, in conjunction With methylation studies supported by fast-atom-bombardment mass spectrometry and linkage analyses by gas chromatography/mass spectrometry. It was concluded on the basis of the following structures, in which each was linked 1-->5 to a terminal 3-deoxy-D-manno-octulosonic acid residue, that the core regions with qualititatively similar sugar compositions showed serotypic differences in one or more of their sequences, linkage types, and anometic configurations: [GRAPHICS] The outer regions of each structure carry Neu5Ac residues linked 2-->3 to available beta-D-Galp residues and show striking similarities with various glycosphingolipids of the ganglioside family. However, Neu5Ac epitopes are not apparently involved in determining serospecificity.
Funder
Publisher
Wiley-Blackwell
Publisher DOI
10.1111/j.1432-1033.1993.tb17849.x
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (1)