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Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector

Mathew, Asha
; 
Cao, Hongliang
; 
Collin, Estelle
; 
Wang, Wenxin
; 
Pandit, Abhay
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Publication Date
2012-06-02
Keywords
2-(Dimethylamino) ethyl methacrylate, Deactivation enhanced atom transfer radical polymerisation, Gene therapy
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Article
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Mathew, A,Cao, HL,Collin, E,Wang, WX,Pandit, A (2012) 'Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector'. International Journal Of Pharmaceutics, 434 :99-105.
Abstract
A unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine) dendrimer (dPAMAM; commercial name: SuperFect (R)) in fibroblasts and adipose tissue derived stem cells (ADSCs). (c) 2012 Elsevier B.V. All rights reserved.
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Publisher
Elsevier
Publisher DOI
DOI 10.1016/j.ijpharm.2012.05.010
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Biomedical Engineering (Scholarly Articles)