Screening of exosomal microRNAs from colorectal cancer cells

Clancy, Cillian
Khan, Sonja
Glynn, Claire L.
Holian, Emma
Dockery, Peter
Lalor, Pierce
Brown, James A.L.
Joyce, Myles R.
Kerin, Michael J.
Dwyer, Róisín M.
Clancy, C,Khan, S,Glynn, CL,Holian, E,Dockery, P,Lalor, P,Brown, JAL,Joyce, MR,Kerin, MJ,Dwyer, RM (2016) 'Screening of exosomal microRNAs from colorectal cancer cells'. Cancer Biomarkers, 17 :427-435, DOI: 10.3233/CBM-160659
BACKGROUND: Cells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown.OBJECTIVE: To identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRNAs.METHODS: Exosomes were isolated from HCT-116 and HT-29 cell lines. RNA was extracted from exosomes and microRNA array performed. Cells were engineered to express miR-379 (HCT-116-379) or a non-targeting control (HCT-116-NTC) and functional effects were determined. Exosomes secreted by engineered cells were transferred to recipient cells and the impact examined.RESULTS: Microvesicles 40-100 nm in size secreted by cell lines were visualised and confirmed to express exosomal protein CD63. HT-29 exosomes contained 409 miRNAs, HCT-116 exosomes contained 393, and 338 were common to exosomes from both cell lines. Selected targets were validated. HCT-116-379 cells showed decreased proliferation (12-15% decrease, p
IOS Press
Publisher DOI
Attribution-NonCommercial-NoDerivs 3.0 Ireland