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Results of a saxitoxin proficiency test including characterization of reference material and stability studies

Harju, Kirsi
; 
Rapinoja, Marja-Leena
; 
Avondet, Marc-André
; 
Arnold, Werner
; 
Schär, Martin
; 
Luginbühl, Werner
; 
Kremp, Anke
; 
Suikkanen, Sanna
; 
Kankaanpää, Harri
; 
Burrell, Stephen
... show 2 more
Repository DOI
Publication Date
2015-11-25
Keywords
paralytic shellfish poisoning toxins, saxitoxin, proficiency test, dinoflagellate, mussel, paralytic shellfish toxins, official method(sm) 2005.06, poisoning toxins, extension, quantification, spectrometry, validation, refinement, improve
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Article
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Harju, Kirsi; Rapinoja, Marja-Leena; Avondet, Marc-André; Arnold, Werner; Schär, Martin; Luginbühl, Werner; Kremp, Anke; Suikkanen, Sanna; Kankaanpää, Harri; Burrell, Stephen; Söderström, Martin; Vanninen, Paula (2015). Results of a saxitoxin proficiency test including characterization of reference material and stability studies. Toxins 7 (12), 4852-4867
Abstract
A saxitoxin (STX) proficiency test (PT) was organized as part of the Establishment of Quality Assurance for the Detection of Biological Toxins of Potential Bioterrorism Risk (EQuATox) project. The aim of this PT was to provide an evaluation of existing methods and the European laboratories' capabilities for the analysis of STX and some of its analogues in real samples. Homogenized mussel material and algal cell materials containing paralytic shellfish poisoning (PSP) toxins were produced as reference sample matrices. The reference material was characterized using various analytical methods. Acidified algal extract samples at two concentration levels were prepared from a bulk culture of PSP toxins producing dinoflagellate Alexandrium ostenfeldii. The homogeneity and stability of the prepared PT samples were studied and found to be fit-for-purpose. Thereafter, eight STX PT samples were sent to ten participating laboratories from eight countries. The PT offered the participating laboratories the possibility to assess their performance regarding the qualitative and quantitative detection of PSP toxins. Various techniques such as official Association of Official Analytical Chemists (AOAC) methods, immunoassays, and liquid chromatography-mass spectrometry were used for sample analyses.
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Publisher
MDPI AG
Publisher DOI
10.3390/toxins7124852
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)