Comparative proteomics implicates a role for multiple secretion systems in electrode-respiring geobacter sulfurreducens biofilms
Kavanagh, Paul Botting, Catherine H. Jana, Partha S. Leech, Dónal Abram, Florence
Kavanagh, Paul
Botting, Catherine H.
Jana, Partha S.
Leech, Dónal
Abram, Florence
Repository DOI
Publication Date
2016-12-02
Type
Article
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Citation
Kavanagh, Paul; Botting, Catherine H. Jana, Partha S.; Leech, Dónal; Abram, Florence (2016). Comparative proteomics implicates a role for multiple secretion systems in electrode-respiring geobacter sulfurreducens biofilms. Journal of Proteome Research 15 (12), 4135-4145
Abstract
Geobacter sulfurreducens is a dissimilatory metal-reducing bacterium capable of forming thick electron-conducting biofilms on solid electrodes. Here we employ for the first time comparative proteomics to identify key physiological changes involved in G. sulfurreducens adaptation from fumarate-respiring planktonic cells to electron-conducting biofilms. Increased levels of proteins involved in outer membrane biogenesis, cell motility, and secretion are expressed in biofilms. Of particular importance to the electron-conducting biofilms are proteins associated with secretion systems of Type I, II, V and Type IV pill. Furthermore, enzymes involved in lipopolysaccharide and peptidoglycan biosynthesis show increased levels of expression in electron-conducting biofilms compared with planktonic cells. These observations point to similarities in long-range electron-transfer mechanisms between G. sulfurreducens and Shewanella oneidensis while highlighting the wider significance of secretion systems beyond that of Type IV pill identified to date in the adaptation of G. sulfurreducens to electrode respiration.
Funder
Publisher
American Chemical Society (ACS)
Publisher DOI
10.1021/acs.jproteome.5b01019
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Attribution-NonCommercial-NoDerivs 3.0 Ireland