Publication

Fe 3 o 4 nanoparticles on graphene oxide sheets for isolation and ultrasensitive amperometric detection of cancer biomarker proteins

Sharafeldin, Mohamed
; 
Bishop, Gregory W.
; 
Bhakta, Snehasis
; 
El-Sawy, Abdelhamid
; 
Suib, Steven L.
; 
Rusling, James F.
Repository DOI
Publication Date
2017-05-01
Keywords
iron oxide, magnetic, graphene, biomarker proteins, immunoarray, microfluidics, peroxidase-like activity, microfluidic immunoassays, immunosorbent-assay, membrane antigen, nanocomposites, glucose, h2o2, quantitation, sensitivity, diagnostics
Type
Article
Downloads
Show all metadata
Citation
Sharafeldin, Mohamed; Bishop, Gregory W. Bhakta, Snehasis; El-Sawy, Abdelhamid; Suib, Steven L.; Rusling, James F. (2017). Fe 3 o 4 nanoparticles on graphene oxide sheets for isolation and ultrasensitive amperometric detection of cancer biomarker proteins. Biosensors and Bioelectronics 91 , 359-366
Abstract
Ultrasensitive mediator-free electrochemical detection for biomarker proteins was achieved at low cost using a novel composite of Fe3O4 nanoparticles loaded onto graphene oxide (GO) nano-sheets (Fe3O4@GO). This paramagnetic Fe3O4@GO composite (1 gm size range) was decorated with antibodies against prostate specific antigen (PSA) and prostate specific.membrane antigen (PSMA), and then used to first capture these biomarkers and then deliver them to an 8-sensor detection chamber of a microfluidic immunoarray. Screen-printed carbon sensors coated with electrochemically reduced graphene oxide (ERGO) and a second set of antibodies selectively capture the biomarker-laden Fe3O4@GO particles, which subsequently catalyze hydrogen peroxide reduction to detect PSA and PSMA. Accuracy was confirmed by good correlation between patient serum assays and enzyme linked immuno-sorbent assays (ELISA). Excellent detection limits (LOD) of 15 fg/mL for PSA and 4.8 fg/mL for PSMA were achieved in serum. The LOD for PSA was 1000-fold better than the only previous report of PSA detection using Fe3O4. Dynamic ranges were easily tunable for concentration ranges encountered in serum samples by adjusting the Fe3O4@GO Concentration. Reagent cost was only $0.85 for a single 2-protein assay.
Funder
Publisher
Elsevier BV
Publisher DOI
10.1016/j.bios.2016.12.052
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
cbn
Collections
Externally hosted open access publications with University of Galway authors (2)