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Carvacrol inhibits osteoclastogenesis and negatively regulates the survival of mature osteoclasts

Deepak, Vishwa
; 
Kasonga, Abe
; 
Kruger, Marlena Cathorina
; 
Coetzee, Magdalena
Repository DOI
Publication Date
2016-01-01
Keywords
osteoclast, osteoporosis, periodontitis, receptor activator of nuclear factor-kappa b ligand (rankl), lipopolysaccharide (lps), nf-kappa-b, bone-resorption, tnf-alpha, in-vitro, lipopolysaccharide, apoptosis, cells, periodontitis, il-1-beta, il-8
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Article
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Citation
Deepak, Vishwa; Kasonga, Abe; Kruger, Marlena Cathorina; Coetzee, Magdalena (2016). Carvacrol inhibits osteoclastogenesis and negatively regulates the survival of mature osteoclasts. Biological and Pharmaceutical Bulletin 39 (7), 1150-1158
Abstract
Bone is a dynamic tissue that undergoes continuous remodeling coupled with the action of osteoblasts and osteoclasts. Osteoclast activity is elevated during osteoporosis and periodontitis resulting in excessive loss of trabecular and alveolar bone. Osteoclasts are formed in an inflammatory response to cytokine production receptor activator of nuclear factor-kappaB (NF-kappa B) ligand (RANKL) and bacterial challenge lipopolysaccharide (LPS). Carvacrol, a monoterpenic phenol present in Origanuin vulgare and Thymus vulgaris, is a natural compound with known medicinal properties. We investigated the effects of carvacrol on osteoclast formation induced by RANKL and LPS. Carvacrol suppressed RANKL-induced formation of tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells in RAW264.7 macrophages and human CD14(+) monocytes. Furthermore, carvacrol inhibited LPS-induced osteoclast formation in RAW264.7 macrophages. Investigation of the underlying molecular mechanisms revealed that carvacrol downregulated RANKL-induced NF-kappa B activation in a dose-dependent manner. Furthermore, the suppression of NF-kappa B activation correlated with inhibition of inhibitor of kappaB (I kappa B) kinase (IKK) activation and attenuation of inhibitor of NF-kappa B (I kappa Ba) degradation. Carvacrol potentiated apoptosis in mature osteoclasts by caspase-3 activation and DNA fragmentation. Moreover, carvacrol did not affect the viability of proliferating MC3T3-E1 osteoblast-like cells. Collectively, these results demonstrate that carvacrol mitigates osteoclastogenesis by impairing the NF-kappa B pathway and induction of apoptosis in mature osteoclasts.
Funder
Publisher
Pharmaceutical Society of Japan
Publisher DOI
10.1248/bpb.b16-00117
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
cbn
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Externally hosted open access publications with University of Galway authors (2)