Construction of synthetic nucleoli and what it tells us about propagation of sub-nuclear domains through cell division
Grob, Alice ; McStay, Brian
Grob, Alice
McStay, Brian
Repository DOI
Publication Date
2014-08-18
Keywords
cell cycle, mitotic bookmarking, nuclear bodies, nucleolus, nucleolar organizer region (nor), neo-nor, neonucleoli, pseudo-nor, synthetic biology, ubf, 1 degrees, primary, 2 degrees, secondary, cbs, cajal bodies, cdk, cyclin-dependent kinase, dfc, dense fibrillar component, dj, distal junction, fcs, fibrillar centers, gc, granular component, hlbs, histone locus bodies, igs, intergenic spacers, hmg, high mobility group, nbs, nuclear bodies, nors, nucleolar organizer regions, pj, proximal junction, pml, promyelocytic leukemia, pnbs, pre-nucleolar bodies, pol, rna polymerase, pre-rrna, precursor rrna, rdna, ribosomal genes, rrna, ribosomal rna, rnp, ribonucleoprotein, tfs, transcription factors, t-utps, transcription u 3 proteins, ubf, upstream binding factor, xen, xenopus enhancer, i transcription factor, ribosomal-rna genes, organizer regions, mammalian-cells, high-mobility, active genes, factor hubf, DNA, body, ubf
Type
Article
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Citation
Grob, Alice; McStay, Brian (2014). Construction of synthetic nucleoli and what it tells us about propagation of sub-nuclear domains through cell division. Cell Cycle 13 (16), 2501-2508
Abstract
The cell nucleus is functionally compartmentalized into numerous membraneless and dynamic, yet defined, bodies. The cell cycle inheritance of these nuclear bodies (NBs) is poorly understood at the molecular level. In higher eukaryotes, their propagation is challenged by cell division through an open mitosis, where the nuclear envelope disassembles along with most NBs. A deeper understanding of the mechanisms involved can be achieved using the engineering principles of synthetic biology to construct artificial NBs. Successful biogenesis of such synthetic NBs demonstrates knowledge of the basic mechanisms involved. Application of this approach to the nucleolus, a paradigm of nuclear organization, has highlighted a key role for mitotic bookmarking in the cell cycle propagation of NBs.
Funder
Publisher
Informa UK Limited
Publisher DOI
10.4161/15384101.2014.949124
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland