Publication

Structures of the o-specific polysaccharide chains of pectinatus cerevisiiphilus and pectinatus frisingensis lipopolysaccharides

Senchenkova, Sof'ya N.
Shashkov, Alexander S.
Moran, Antony P.
Helander, Ilkka M.
Knirel, Yuriy A.
Citation
Senchenkova, Sof'ya N. Shashkov, Alexander S.; Moran, Antony P.; Helander, Ilkka M.; Knirel, Yuriy A. (1995). Structures of the o-specific polysaccharide chains of pectinatus cerevisiiphilus and pectinatus frisingensis lipopolysaccharides. European Journal of Biochemistry 232 (2), 552-557
Abstract
Mild acid hydrolysis of the smooth-type lipopolysaccharide (LPS) of Pectinatus frisingensis afforded no polysaccharide but monomeric 6-deoxy-L-altrose (L-6dAIt) which was identified by anion-exchange chromatography in berate buffer, GLC/MS, H-1-NMR spectroscopy, and optical rotation. LPS was degraded with alkali under reductive conditions to give a completely O-deacylated polysaccharide, which was studied by methylation analysis, H-1-NMR and C-13-NMR spectroscopy, including sequential, selective spin-decoupling, two-dimensional correlation spectroscopy (COSY), COSY with relayed coherence transfer, two-dimensional heteronuclear C-13, H-1-COSY, one-dimensional NOE and two-dimensional rotating-frame NOE spectroscopy. It was found that the O-specific polysaccharide chain of P. frisingensis LPS is a homopolymer of 6-deoxy-L-altrofuranose built up of tetrasaccharide-repeating units having the following structure: [GRAPHICS] Similarly, mild acid degradation of smooth-type LPS of Pectinatus cerevisiiphilus resulted in depolymerisation of the polysaccharide chain to give a disaccharide consisting of D-glucose and D-fucose, Study of the disaccharide by methylation analysis and alkali-degraded LPS by one-dimensional and two-dimensional H-1-NMR and C-13-NMR spectroscopy showed that the O-specific polysaccharide of P. cerevisiiphilus has the following structure: -->2)-beta-D-Fucf-(1-->2)-alpha-D-Glcp-(1-->
Funder
Publisher
Wiley-Blackwell
Publisher DOI
10.1111/j.1432-1033.1995.552zz.x
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland