Publication

Structural characterization of neutral oligosaccharides with blood-group a and h activity isolated from bovine submaxillary mucin

Savage, A V
D'Arcy, S M
Donoghue, C M
Citation
Savage, A V; D'Arcy, S M; Donoghue, C M (1991). Structural characterization of neutral oligosaccharides with blood-group a and h activity isolated from bovine submaxillary mucin. Biochemical Journal 279 , 95-103
Abstract
In this study we investigated the structures of 11 neutral oligosaccharides released from bovine submaxillary mucin by alkaline borohydride treatment and isolated by h.p.l.c. One hexa-, one penta-, three tetra-, four tri- and two di-saccharides containing core types 1, 2, 3 or 4 were obtained. We report their structures, determined by a combination of one- and two-dimensional H-1 n.m.r. spectroscopy at 270 MHz and methylation analysis involving g.l.c.-m.s., along with their approximate molar ratios. Only three of these oligosaccharides have previously been reported in this source. Of the new oligosaccharides, one contains the blood-group-A antigenic determinant, two contain the blood-group-H type 2 determinant, while another contains the blood-group-H type 3 determinant. The oligosaccharide GlcNAc-beta(1 --> 6)[GlcNAc-beta(1 --> 3)]GalNAcol, although previously found as a core structure, has been isolated here as a novel trisaccharide.
Funder
Publisher
Portland Press Ltd.
Publisher DOI
10.1042/bj2790095
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland