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Structural characterization of neutral oligosaccharides with blood-group a and h activity isolated from bovine submaxillary mucin

Savage, A V
; 
D'Arcy, S M
; 
Donoghue, C M
Identifiers
http://hdl.handle.net/10379/9844
 https://doi.org/10.13025/23826
Publication Date
1991-10-01
Keywords
500-mhz h-1-nmr spectroscopy, pressure liquid-chromatography, respiratory-mucus glycoproteins, quantitative sugar analysis, acidic oligosaccharide, carbohydrate chains, cystic-fibrosis, gland mucin, core type-1, combination
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Article
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Citation
Savage, A V; D'Arcy, S M; Donoghue, C M (1991). Structural characterization of neutral oligosaccharides with blood-group a and h activity isolated from bovine submaxillary mucin. Biochemical Journal 279 , 95-103
Abstract
In this study we investigated the structures of 11 neutral oligosaccharides released from bovine submaxillary mucin by alkaline borohydride treatment and isolated by h.p.l.c. One hexa-, one penta-, three tetra-, four tri- and two di-saccharides containing core types 1, 2, 3 or 4 were obtained. We report their structures, determined by a combination of one- and two-dimensional H-1 n.m.r. spectroscopy at 270 MHz and methylation analysis involving g.l.c.-m.s., along with their approximate molar ratios. Only three of these oligosaccharides have previously been reported in this source. Of the new oligosaccharides, one contains the blood-group-A antigenic determinant, two contain the blood-group-H type 2 determinant, while another contains the blood-group-H type 3 determinant. The oligosaccharide GlcNAc-beta(1 --> 6)[GlcNAc-beta(1 --> 3)]GalNAcol, although previously found as a core structure, has been isolated here as a novel trisaccharide.
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Publisher
Portland Press Ltd.
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
cbn
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Externally hosted open access publications with University of Galway authors (1)