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Nucleolar activity and cenp-c regulate cenp-a and cal1 availability for centromere assembly in meiosis

Kwenda, Lucretia
; 
Collins, Caitriona M.
; 
Dattoli, Anna A.
; 
Dunleavy, Elaine M.
Repository DOI
Publication Date
2016-04-15
Keywords
centromere, meiosis, spermatogenesis, cenp-a, cid, nucleolus, sister-chromatid cohesion, drosophila-melanogaster, epigenetic regulation, temporal control, maintenance, mitosis, spermatogenesis, transcription, segregation, propagation
Type
Article
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Citation
Kwenda, Lucretia; Collins, Caitriona M. Dattoli, Anna A.; Dunleavy, Elaine M. (2016). Nucleolar activity and cenp-c regulate cenp-a and cal1 availability for centromere assembly in meiosis. Development 143 (8), 1400-1412
Abstract
The centromere-specific histone CENP-A is the key epigenetic determinant of centromere identity. Whereas most histones are removed from mature sperm, CENP-A is retained to mark paternal centromeres. In Drosophila males we show that the centromere assembly factors CAL1 and CENP-C are required for meiotic chromosome segregation, CENP-A assembly and maintenance on sperm, as well as fertility. In meiosis, CENP-A accumulates with CAL1 in nucleoli. Furthermore, we show that CENP-C normally limits the release of CAL1 and CENP-A from nucleoli for proper centromere assembly in meiotic prophase I. Finally, we show that RNA polymerase I transcription is required for efficient CENP-A assembly in meiosis, as well as centromere tethering to nucleoli.
Funder
Publisher
The Company of Biologists
Publisher DOI
10.1242/dev.130625
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
cbn
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Externally hosted open access publications with University of Galway authors (2)