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Detection of salmonid alphavirus rna in celtic and irish sea flatfish

McCleary, S
; 
Giltrap, M
; 
Henshilwood, K
; 
Ruane, NM
Repository DOI
Publication Date
2014-04-23
Keywords
sav, pancreas disease, wild fish, horizontal transmission, atlantic salmon, aquaculture, farmed atlantic salmon, pancreas disease virus, wild marine fish, salar l., molecular epidemiology, nucleotide-sequences, north-atlantic, ireland, aquaculture, waters
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Article
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Citation
McCleary, S; Giltrap, M; Henshilwood, K; Ruane, NM (2014). Detection of salmonid alphavirus rna in celtic and irish sea flatfish. Diseases of Aquatic Organisms 109 (1), 1-7
Abstract
Pancreas disease (PD) caused by the salmonid alphavirus (SAV) has been the most significant cause of mortalities in Irish farmed salmon Salmo salar L. over the past decade. SAV is a single-strand positive-sense RNA virus, originally thought to be unique to salmonids, but has recently been detected using real-time RT-PCR in a number of wild non-salmonid fish. In the present report, 610 wild flatfish (common dab Limanda limanda, plaice Pleuronectes platessa and megrim Lepidorhombus whiffiagonis) were caught from the Irish and Celtic Seas and screened for SAV using real-time RT-PCR and sequencing. In general, a very low prevalence was recorded in common dab and plaice, except for 1 haul in Dublin Bay where 25% of common dab were SAV-positive. SAV sequence analysis supported the fact that real-time RT-PCR detections were specific and further characterised the detected viruses within SAV Subtype I, the predominant subtype found in farmed salmon in Ireland.
Funder
Publisher
Inter-Research Science Center
Publisher DOI
10.3354/dao02719
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)