Elicitation of resistance and associated defense responses in trichoderma hamatum induced protection against pearl millet downy mildew pathogen
Siddaiah, Chandra Nayaka Satyanarayana, Niranjan Raj Mudili, Venkataramana Kumar Gupta, Vijai Gurunathan, Selvakumar Rangappa, Shobith Huntrike, Shekar Shetty Srivastava, Rakesh Kumar
Siddaiah, Chandra Nayaka
Satyanarayana, Niranjan Raj
Mudili, Venkataramana
Kumar Gupta, Vijai
Gurunathan, Selvakumar
Rangappa, Shobith
Huntrike, Shekar Shetty
Srivastava, Rakesh Kumar
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http://hdl.handle.net/10379/13904
https://doi.org/10.13025/26390
https://doi.org/10.13025/26390
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Publication Date
2017-03-21
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Article
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Siddaiah, Chandra Nayaka; Satyanarayana, Niranjan Raj; Mudili, Venkataramana; Kumar Gupta, Vijai; Gurunathan, Selvakumar; Rangappa, Shobith; Huntrike, Shekar Shetty; Srivastava, Rakesh Kumar (2017). Elicitation of resistance and associated defense responses in trichoderma hamatum induced protection against pearl millet downy mildew pathogen. Scientific Reports 7 ,
Abstract
Endophytic Trichoderma hamatum UoM 13 isolated from pearl millet roots was evaluated for its efficiency to suppress downy mildew disease. Under laboratory conditions, T. hamatum seed treatment significantly enhanced pearl millet seed germination and seedling vigor. T. hamatum seed treatment resulted in systemic and durable immunity against pearl millet downy mildew disease under greenhouse and field conditions. T. hamatum treated seedlings responded to downy mildew infection with high lignification and callose deposition. Analysis of defense enzymes showed that T. hamatum treatment significantly enhanced the activities of glucanase, peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase in comparison to untreated control. RT-PCR analysis revealed differentially expressed transcripts of the defense enzymes and PR-proteins in treated, untreated, and checks, wherein PR-1, PR-5, and cell wall defense HRGPs were significantly over expressed in treated seedlings as against their lower expression in controls. T. hamatum treatment significantly stimulated endogenous salicylic acid ( SA) levels and significantly upregulated important SA biosynthesis gene isochorismate synthase. The results indicated that T. hamatum UoM13 treatment induces resistance corresponding to significant over expression of endogenous SA, important defense enzymes, PR-proteins, and HRGPs, suggesting that SA biosynthetic pathway is involved in pearl millet for mounting systemic immunity against downy mildew pathogen.
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Springer Nature
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Attribution-NonCommercial-NoDerivs 3.0 Ireland