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Differences in the expression of genes involved in the somatotropic axis in divergent strains of holstein-friesian dairy cows during early and mid lactation

McCarthy, S.D.
; 
Butler, S.T.
; 
Patton, J.
; 
Daly, M.
; 
Morris, D.G.
; 
Kenny, D.A.
; 
Waters, S.M.
Repository DOI
Publication Date
2009-10-01
Keywords
holstein-friesian, liver, gene expression, somatotropic axis, growth-factor-i, negative-energy balance, acid-labile subunit, messenger-ribonucleic-acid, factor-binding protein-2, perfused-rat-liver, hormone receptor, transcription factors, milk-production, gh responsiveness
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Article
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Citation
McCarthy, S.D. Butler, S.T.; Patton, J.; Daly, M.; Morris, D.G.; Kenny, D.A.; Waters, S.M. (2009). Differences in the expression of genes involved in the somatotropic axis in divergent strains of holstein-friesian dairy cows during early and mid lactation. Journal of Dairy Science 92 (10), 5229-5238
Abstract
Differences in genetic selection criteria for dairy cows internationally have led to divergence in the Holstein-Friesian breed. The objective of this study was to compare hepatic expression of genes of the somatotropic axis in the North American Holstein-Friesian and the New Zealand Holstein-Friesian strains of dairy cow at early and mid lactation. Mature cows of both the North American Holstein-Friesian (n = 10) and New Zealand Holstein- Friesian (n = 10) strains were selected. Liver tissue was collected by percutaneous punch biopsy from all cows at 35 and 140 d postpartum, representing early and mid lactation, respectively. Total RNA was extracted and the hepatic expression of genes involved in the control of the somatotropic axis was examined. Abundance of insulin-like growth factor (IGF)-1 mRNA was greater in the New Zealand strain, concomitant with a tendency for increased expression of acid-labile subunit mRNA. Across strains, mRNA abundance of IGF-binding protein-1, IGF-binding protein-2, and growth hormone receptor 1A decreased from d 35 to 140 postpartum, whereas expression of IGF-1 and acid-labile subunit tended to increase. Abundance of suppressor of cytokine signaling-3 mRNA was increased at d 140 postpartum. Both the strain of Holstein- Friesian cow and the stage of lactation influenced expression of genes controlling the somatotropic axis in hepatic tissue.
Funder
Publisher
American Dairy Science Association
Publisher DOI
10.3168/jds.2008-1856
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)