Publication

The budding yeast rad9 checkpoint complex: chaperone proteins are required for its function

Gilbert, Christopher S
van den Bosch, Michael
Green, Catherine M
Vialard, Jorge E
Grenon, Muriel
Erdjument-Bromage, Hediye
Tempst, Paul
Lowndes, Noel F
Citation
Gilbert, Christopher S; van den Bosch, Michael; Green, Catherine M; Vialard, Jorge E; Grenon, Muriel; Erdjument-Bromage, Hediye; Tempst, Paul; Lowndes, Noel F (2003). The budding yeast rad9 checkpoint complex: chaperone proteins are required for its function. EMBO reports 4 (10), 953-958
Abstract
Rad9 functions in the DNA-damage checkpoint pathway of Saccharomyces cerevisiae. In whole-cell extracts, Rad9 is found in large, soluble complexes, which have functions in amplifying the checkpoint signal. The two main soluble forms of Rad9 complexes that are found in cells exposed to DNA-damaging treatments were purified to homogeneity. Both of these Rad9 complexes contain the Ssa1 and/or Ssa2 chaperone proteins, suggesting a function for these proteins in checkpoint regulation. Consistent with this possibility, genetic experiments indicate redundant functions for SSA1 and SSA2 in survival, G2/M-checkpoint regulation, and phosphorylation of both Rad9 and Rad53 after irradiation with ultraviolet light. Ssa1 and Ssa2 can now be considered as novel checkpoint proteins that are likely to be required for remodelling Rad9 complexes during checkpoint-pathway activation.
Funder
Publisher
Wiley-Blackwell
Publisher DOI
10.1038/sj.embor.embor935
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland