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Restriction endonuclease mapping of the hi2 incompatibility group plasmid r478.

Whelan, K F
; 
Colleran, E
Identifiers
http://hdl.handle.net/10379/9980
 https://doi.org/10.13025/23932
Publication Date
1992-02-01
Keywords
escherichia-coli, chloramphenicol resistance, bacterial plasmids, typhoid bacillus, transfer regions, r-plasmids, anion pump, DNA, strains, r27
Type
Article
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Citation
Whelan, K F; Colleran, E (1992). Restriction endonuclease mapping of the hi2 incompatibility group plasmid r478.. Journal of Bacteriology 174 (4), 1197-1204
Abstract
A restriction map of the 272-kb IncHI2 plasmid R478 was constructed by using the enzymes ApaI, XbaI, SalI, and XhoI. The map was derived from cloned restriction fragments from R478 inserted into cosmid and plasmid vectors as well as from double-digestion analysis of R478 and R478 miniplasmids. All previously known resistance determinants were cloned from R478, and their positions were located on the restriction map. A region involved in incompatibility was cloned and mapped. The location of a previously unreported arsenite resistance gene was also determined. The genes encoding tellurite resistance, colicin B resistance, and phage inhibition were found to be associated with a 6.7-kb SalI fragment of R478.
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Publisher
American Society for Microbiology
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (1)