Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.)
Davey, Grace Houeix, Benoit Talbot, Anita Cairns, Michael T.
Davey, Grace
Houeix, Benoit
Talbot, Anita
Cairns, Michael T.
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Publication Date
2010-03-22
Type
Article
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Citation
Calduch-Giner, JA,Davey, G,Saera-Vila, A,Houeix, B,Talbot, A,Prunet, P,Cairns, MT,Perez-Sanchez, J (2010) 'Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.)'. BMC Genomics, 11 .
Abstract
Background: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream ( Sparus aurata) is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement of this species. With the aim of increasing the genomic resources in gilthead sea bream and identifying genes and mechanisms underlying the physiology of the stress response, we developed a cDNA microarray for gilthead sea bream that is enriched by suppression substractive hybridization with stress and immunorelevant genes. This microarray is used to analyze the dynamics of gilthead sea bream liver expression profile after confinement exposure.Results: Groups of confined and control juvenile fish were sampled at 6, 24, 72 and 120 h post exposure. GeneSpring analyses identified 202 annotated genes that appeared differentially expressed at least at one sampling time (P
Funder
Publisher
BioMed Central
Publisher DOI
10.1186/1471-2164-11-193
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Attribution-NonCommercial-NoDerivs 3.0 Ireland