High-resolution imaging of ph in alkaline sediments and water based on a new rapid response fluorescent planar optode
Han, Chao Yao, Lei Xu, Di Xie, Xianchuan Zhang, Chaosheng
Han, Chao
Yao, Lei
Xu, Di
Xie, Xianchuan
Zhang, Chaosheng
Publication Date
2016-05-20
Type
Article
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Han, Chao; Yao, Lei; Xu, Di; Xie, Xianchuan; Zhang, Chaosheng (2016). High-resolution imaging of ph in alkaline sediments and water based on a new rapid response fluorescent planar optode. Scientific Reports 6 ,
Abstract
A new dual-lumophore optical sensor combined with a robust RGB referencing method was developed for two-dimensional (2D) pH imaging in alkaline sediments and water. The pH sensor film consisted of a proton-permeable polymer (PVC) in which two dyes with different pH sensitivities and emission colors: (1) chloro phenyl imino propenyl aniline (CPIPA) and (2) the coumarin dye Macrolex (R) fluorescence yellow 10 GN (MFY-10 GN) were entrapped. Calibration experiments revealed the typical sigmoid function and temperature dependencies. This sensor featured high sensitivity and fast response over the alkaline working ranges from pH 7.5 to pH 10.5. Cross-sensitivity towards ionic strength (IS) was found to be negligible for freshwater when IS <0.1 M. The sensor had a spatial resolution of approximately 22 mu m and aresponse time of <120 s when going from pH 7.0 to 9.0. The feasibility of the sensor was demonstrated using the pH microelectrode. An example of pH image obtained in the natrual freshwater sediment and water associated with the photosynthesis of Vallisneria spiral species was also presented, suggesting that the sensor held great promise for the field applications.
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Publisher
Springer Nature
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Attribution-NonCommercial-NoDerivs 3.0 Ireland