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Evaluation of a novel PCR based diagnostic assay for the detection of Mycobacterium tuberculosis in sputum specimens

Maher, Majella
; 
Glennon, Maura
; 
Smith, Terry
; 
Dawson, Michael T.
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Identifiers
http://hdl.handle.net/10379/4041
 https://doi.org/10.13025/21612
Publication Date
1996-06-18
Keywords
Polymerase chain-reaction, Clinical samples, DNA amplification, Identification, Specimens, Sediments
Type
Article
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Citation
Maher, M., Glennon, M., Martinazzo, E., Turchetti, E., Marcolini, S., Smith, T.J. and Dawson, M.T. (1996) 'Evaluation of a novel PCR based diagnostic assay for the detection of Mycobacterium tuberculosis in sputum specimens'. JOURNAL OF CLINICAL MICROBIOLOGY, 34 (9):2307-2308.
Abstract
We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.
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Publisher
ACM
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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National Centre for Biomedical Engineering Science (Scholarly Articles)