Publication

Amine functionalization of cholecyst-derived extracellular matrix with generation 1 pamam dendrimer

Chan, Jeffrey C. Y.
; 
Burugapalli, Krishna
; 
Naik, Hemantkumar
; 
Kelly, John L.
; 
Pandit, Abhay
Identifiers
http://hdl.handle.net/10379/10740
https://doi.org/10.13025/26972
Repository DOI
10.1021/bm701055k
Publication Date
2008-02-01
Keywords
cross-linked collagen, small-intestinal submucosa, dermal sheep collagen, tissue engineering applications, growth-factor delivery, body-wall repair, abdominal-wall, regenerating capacity, biological stability, physical-properties
Type
Article
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Citation
Chan, Jeffrey C. Y. Burugapalli, Krishna; Naik, Hemantkumar; Kelly, John L.; Pandit, Abhay (2008). Amine functionalization of cholecyst-derived extracellular matrix with generation 1 pamam dendrimer. Biomacromolecules 9 (2), 528-536
Abstract
A method to functionalize cholecyst-derived extracellular matrix (CEM) with free amine groups was established in an attempt to improve its potential for tethering of bioactive molecules. CEM was incorporated with Generation-1 polyamidoamine (G1 PAMAM) dendrimer by using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysuccinimide cross-linking system. The nature of incorporation of PAMAM dendrimer was evaluated using shrink temperature measurements, Fourier transform infrared (FTIR) assessment, ninhydrin assay, and swellability. The effects of PAMAM incorporation on mechanical and degradation properties of CEM were evaluated using a uniaxial mechanical test and collagenase degradation assay, respectively. Ninhydrin assay and FTIR assessment confirmed the presence of increasing free amine groups with increasing quantity of PAMAM in dendrimer-incorporated CEM (DENCEM) scaffolds. The amount of dendrimer used was found to be critical in controlling scaffold degradation, shrink temperature, and free amine content. Cell culture studies showed that fibroblasts seeded on DENCEM maintained their metabolic activity and ability to proliferate in vitro. In addition, fluorescence cell staining and scanning electron microscopy analysis of cell-seeded DENCEM showed preservation of normal fibroblast morphology and phenotype.
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Publisher
American Chemical Society (ACS)
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)