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Antimicrobial biosynthetic potential and genetic diversity of endophytic actinomycetes associated with medicinal plants

Gohain, Anwesha
; 
Gogoi, Animesh
; 
Debnath, Rajal
; 
Yadav, Archana
; 
Singh, Bhim P.
; 
Gupta, Vijai K.
; 
Sharma, Rajeev
; 
Saikia, Ratul
Identifiers
http://hdl.handle.net/10379/11684
 https://doi.org/10.13025/27006
Publication Date
2015-09-06
Keywords
endophytic actinomycetes, 16s rrna gene, biosynthetic gene, polyketide synthase, sp-nov., functional diversity, natural-products, rain-forests, streptomyces, china, soil, actinobacteria, instability, sediment
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Article
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Gohain, Anwesha; Gogoi, Animesh; Debnath, Rajal; Yadav, Archana; Singh, Bhim P. Gupta, Vijai K.; Sharma, Rajeev; Saikia, Ratul (2015). Antimicrobial biosynthetic potential and genetic diversity of endophytic actinomycetes associated with medicinal plants. FEMS Microbiology Letters 362 (19),
Abstract
Endophytic actinomycetes are one of the primary groups that share symbiotic relationships with medicinal plants and are key reservoir of biologically active compounds. In this study, six selective medicinal plants were targeted for the first time for endophytic actinomycetes isolation from Gibbon Wild Life Sanctuary, Assam, India, during winter and summer and 76 isolates were obtained. The isolates were found to be prevalent in roots followed by stem and leaves. 16S rRNA gene sequence analysis revealed 16 genera, including rare genera, Verrucosispora, Isoptericola and Kytococcus, which have never been previously reported as endophytic. The genus Streptomyces (66%) was dominant in both seasons. Shannon's diversity index showed that Azadirachta indica (1.49), Rauwolfia serpentina (1.43) and Emblica officinalis (1.24) were relatively good habitat for endophytic actinomycetes. Antimicrobial strains showed prevalence of polyketide synthase (PKS) type-II (85%) followed by PKS type-I (14%) encoded in the genomes. Expression studies showed 12-fold upregulation of PKSII gene in seventh day of incubation for Streptomyces antibioticus (EAAG90). Our results emphasize that the actinomycetes assemblages within plant tissue exhibited biosynthetic systems encoding for important biologically active compounds.
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Oxford University Press (OUP)
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)