A Fluorescently Labeled, Hyperbranched Polymer Synthesized from DE-ATRP for the Detection of DNA Hybridization
Aied, Ahmed Glynn, Barry Cao, Hongliang Zheng, Yu Pandit, Abhay Wang, Wenxin
Aied, Ahmed
Glynn, Barry
Cao, Hongliang
Zheng, Yu
Pandit, Abhay
Wang, Wenxin
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Publication Date
2012-01
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Article
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Aied, A,Glynn, B,Cao, HL,Zheng, Y,Tai, HY,Pandit, A,Wang, WX (2012) 'A Fluorescently Labeled, Hyperbranched Polymer Synthesized from DE-ATRP for the Detection of DNA Hybridization'. Polymer Chemistry, 3 :332-334.
Abstract
The early detection of oligonucleotide biomarkers of disease, such as microRNAs, has been established as a fundamental factor in cancer diagnosis. As the levels of these small molecules (microRNAs) in blood have recently been found to be significantly affected in cancer patients, they offer a means of early stage detection of cancer. Towards the goal of creating a novel method of DNA hybridization detection, we report the detection of specific sequences of small oligonucleotides in a model experiment carried out in serum. The results shown here display the versatility of the DE-ATRP method in synthesizing a specific polymer structure capable of changing its physical properties in the presence of double stranded DNA. The polymer was labeled and used to detect single-stranded DNA in serum successfully.
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Royal Society of Chemistry
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Attribution-NonCommercial-NoDerivs 3.0 Ireland