Initiation of jc virus dna replication in vitro by human and mouse dna polymerase alpha-primase
Smith, Richard W. P. Nasheuer, Heinz-Peter
Smith, Richard W. P.
Nasheuer, Heinz-Peter
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Publication Date
2003-05-01
Keywords
DNA replication, initiation, DNA polymerase alpha-primase, species specificity, polyomavirus, human polyomavirus-jc, large t-antigen, progressive multifocal leukoencephalopathy, species-specific replication, functional-characterization, regulatory region, complex-formation, sv40 origin, p48 control, host-range
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journal article
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Citation
Smith, Richard W. P. Nasheuer, Heinz-Peter (2003). Initiation of jc virus dna replication in vitro by human and mouse dna polymerase alpha-primase. European Journal of Biochemistry 270 (9), 2030-2037
Abstract
Host species specificity of the polyomaviruses simian virus 40 (SV40) and mouse polyomavirus (PyV) has been shown to be determined by the host DNA polymerase alpha-primase complex involved in the initiation of both viral and host DNA replication. Here we demonstrate that DNA replication of the related human pathogenic polyomavirus JC virus (JCV) can be supported in vitro by DNA polymerase alpha-primase of either human or murine origin indicating that the mechanism of its strict species specificity differs from that of SV40 and PyV. Our results indicate that this may be due to differences in the interaction of JCV and SV40 large T antigens with the DNA replication initiation complex.
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Publisher
Wiley-Blackwell