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Venom extraction method influences venom composition and potency in the giant house spider Eratigena atrica (C. L. Koch, 1843)

Lyons, Keith
; 
Dugon, Michel M.
; 
Boyd, Aoife
; 
Healy, Kevin
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Identifiers
https://hdl.handle.net/10379/19157
 https://doi.org/10.13025/29951
Publication Date
2023-09-28
Keywords
Microbiology, Extraction, Spider, Composition, PotencyED50, LD50
Type
journal article
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Citation
Lyons, Keith, Dugon, Michel M., Boyd, Aoife, & Healy, Kevin. (2023). Venom extraction method influences venom composition and potency in the giant house spider Eratigena atrica (C. L. Koch, 1843). Toxicon, 234, 107303. https://doi.org/10.1016/j.toxicon.2023.107303
Abstract
Extraction is the first step when investigating venom composition and function. In small invertebrates, widely used extraction methods include electrostimulation and venom gland extraction, however, the influence of these methods on composition and toxicology is poorly understood. Using the Giant House Spider Eratigena atrica as a model, we show that electrostimulation and venom gland removal extraction methods produce different protein profiles as assessed by Coomassie-stained SDS-PAGE and significantly different potencies in the cricket Acheta domesticus.
Funder
Irish Research Council
Thomas Crawford Hayes Research Award, University of Galway
Publisher
Elsevier
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CC BY
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