High-throughput electrochemical microfluidic immunoarray for multiplexed detection of cancer biomarker proteins
Tang, Chi K. Vaze, Abhay Shen, Min Rusling, James F.
Tang, Chi K.
Vaze, Abhay
Shen, Min
Rusling, James F.
Publication Date
2016-08-26
Type
Article
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Citation
Tang, Chi K. Vaze, Abhay; Shen, Min; Rusling, James F. (2016). High-throughput electrochemical microfluidic immunoarray for multiplexed detection of cancer biomarker proteins. ACS Sensors 1 (8), 1036-1043
Abstract
Microchip-based microfluidic electrochemical arrays hold great promise for fast, high-throughput multiplexed detection of cancer biomarker proteins at low cost per assay using relatively simple instrumentation. Here we describe an inexpensive high-throughput electrochemical array featuring 32 individually addressable micro electrodes that is further multiplexed with an 8-port manifold to provide 256 sensors. The gold electrode arrays were fabricated by wet etching commercial gold compact discs (CD-R) followed by patterned insulation. A print-and-peel method was used to create sub-microliter hydrophobic wells surrounding each sensor to eliminate cross contamination during immobilization of capture antibodies. High throughput analyses were realized using eight 32-sensor immunoarrays connected to the miniaturized 8-port manifold, allowing 256 measurements in <1 h. This system was used to determine prostate cancer biomarker proteins prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), interleukin-6 (IL-6), and platelet factor-4 (PF-4) in serum. Clinically relevant detection limits (0.05 to 2 pg mL(-1)) and 5-decade dynamic ranges (sub pg mL(-1) to well above ng mL(-1)) were achieved for these proteins utilizing precapture of analyte proteins on magnetic nanoparticles decorated with enzyme labels and antibodies.
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Publisher
American Chemical Society (ACS)
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Attribution-NonCommercial-NoDerivs 3.0 Ireland