Publication

Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells

Prasad, Babu R.
Mullins, Gillian
Nikolskaya, Natalia
Connolly, David
Smith, Terry
Rochev, Yury
Citation
Prasad, BR,Mullins, G,Nikolskaya, N,Connolly, D,Smith, TJ,Gerard, VA,Byrne, SJ,Davies, GL,Gun'ko, YK,Rochev, Y (2012) 'Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells'. Journal Of Nanobiotechnology, 10 (4).
Abstract
Background: The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy.Results: Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs.Conclusion: The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).
Funder
Publisher
BioMed Central
Publisher DOI
10.1186/1477-3155-10-4
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland