A clinically relevant in vivo model for the assessment of scaffold efficacy in abdominal wall reconstruction
Chan, Jeffrey CY Burugapalli, Krishna Huang, Yi-Shiang Kelly, John L Pandit, Abhay
Chan, Jeffrey CY
Burugapalli, Krishna
Huang, Yi-Shiang
Kelly, John L
Pandit, Abhay
Publication Date
2016-12-30
Keywords
animal model, abdominal wall reconstruction, biologic meshes, dimensional measurement, quantitative stereological analysis, ventral hernia repair, randomized controlled-trial, replacing tissue defects, acellular dermal matrix, incisional hernia, polypropylene-mesh, marlex mesh, expanded polytetrafluoroethylene, adhesion formation, biologic-mesh
Type
Article
Downloads
Citation
Chan, Jeffrey CY; Burugapalli, Krishna; Huang, Yi-Shiang; Kelly, John L; Pandit, Abhay (2016). A clinically relevant in vivo model for the assessment of scaffold efficacy in abdominal wall reconstruction. Journal of Tissue Engineering 8 ,
Abstract
An animal model that allows for assessment of the degree of stretching or contraction of the implant area and the in vivo degradation properties of biological meshes is required to evaluate their performance in vivo. Adult New Zealand rabbits underwent full thickness subtotal unilateral rectus abdominis muscle excision and were reconstructed with the nonbiodegradable Peri-Guard (R), Prolene (R) or biodegradable Surgisis (R) meshes. Following 8 weeks of recovery, the anterior abdominal wall tissue samples were collected for measurement of the implant dimensions. The Peri-Guard and Prolene meshes showed a slight and obvious shrinkage, respectively, whereas the Surgisis mesh showed stretching, resulting in hernia formation. Surgisis meshes showed in vivo biodegradation and increased collagen formation. This surgical rabbit model for abdominal wall defects is advantageous for evaluating the in vivo behaviour of surgical meshes. Implant area stretching and shrinkage were detected corresponding to mesh properties, and histological analysis and stereological methods supported these findings.
Funder
Publisher
SAGE Publications
Publisher DOI
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland