Analysing bovine sperm cells using flow cytometry
Waters, Irene
Waters, Irene
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Publication Date
2024-04-10
Type
Thesis
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Abstract
The accurate assessment of bull fertility is extremely important to the beef and dairy industry. In recent years, flow cytometry has become the dominant approach in sperm evaluation and is increasingly being used for quality assessment and for research in veterinary science. The aim of this research was to show how flow cytometry can be used to assess the quality of semen used in artificial insemination. The review focuses on the methodologies, currently used for bovine sperm concentration assessment and the advantage of flow cytometry has over other methods used for the semen concentration assessment. The research article investigates the technological differences between AccuCell Vs Flow Cytometry to determine semen concentration. It also accesses the reproducibility of results depending on the personnel’s techniques. Based on the analysis of sample diluents’ effect on the results generated with the flow cytometer and estimation of debris’ presence effect in samples, the optimal parameters of ejaculates to determine semen concentration using the flow cytometer are presented. Finally, the sustainability assessment of novel flow cytometry methodology for the semen concentration analysis of an ejaculate demonstrated that flow cytometry is a more sustainable method to use to determine the concentration of an ejaculate.
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Publisher
NUI Galway