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Genome-wide association mapping reveals that specific and pleiotropic regulatory mechanisms fine-tune central metabolism and growth in arabidopsis

Fusari, Corina M.
; 
Kooke, Rik
; 
Lauxmann, Martin A.
; 
Annunziata, Maria Grazia
; 
Encke, Beatrice
; 
Hoehne, Melanie
; 
Krohn, Nicole
; 
Becker, Frank F.M.
; 
Schlereth, Armin
; 
Sulpice, Ronan
... show 2 more
Identifiers
http://hdl.handle.net/10379/11566
https://doi.org/10.13025/27756
Repository DOI
10.1105/tpc.17.00232
Publication Date
2017-09-27
Keywords
quantitative trait loci, adp-glucose pyrophosphorylase, mechanosensitive ion-channel, spatial genetic-structure, enzyme-activities, nitrogen-metabolism, plant development, candidate genes, malate-dehydrogenase, secondary metabolism
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Article
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Citation
Fusari, Corina M. Kooke, Rik; Lauxmann, Martin A.; Annunziata, Maria Grazia; Encke, Beatrice; Hoehne, Melanie; Krohn, Nicole; Becker, Frank F.M.; Schlereth, Armin; Sulpice, Ronan; Stitt, Mark; Keurentjes, Joost J.B. (2017). Genome-wide association mapping reveals that specific and pleiotropic regulatory mechanisms fine-tune central metabolism and growth in arabidopsis. The Plant Cell 29 (10), 2349-2373
Abstract
Central metabolism is a coordinated network that is regulated at multiple levels by resource availability and by environmental and developmental cues. Its genetic architecture has been investigated by mapping metabolite quantitative trait loci (QTL). A more direct approach is to identify enzyme activity QTL, which distinguishes between cis-QTL in structural genes encoding enzymes and regulatory trans-QTL. Using genome-wide association studies, we mapped QTL for 24 enzyme activities, nine metabolites, three structural components, and biomass in Arabidopsis thaiiana. We detected strong cis-QTL for five enzyme activities. A cis-QTL for UDP-glucose pyrophosphorylase activity in the UGP1 promoter is maintained through balancing selection. Variation in acid invertase activity reflects multiple evolutionary events in the promoter and coding region of VAC-INV. cis-QTL were also detected for ADP-glucose pyrophosphorylase, fumarase, and phosphoglucose isomerase activity. We detected many trans-QTL, including transcription factors, E3 ligases, protein targeting components, and protein kinases, and validated some by knockout analysis. trans-QTL are more frequent but tend to have smaller individual effects than cis-QTL. We detected many colocalized QTL, including a multitrait QTL on chromosome 4 that affects six enzyme activities, three metabolites, protein, and biomass. These traits are coordinately modified by different ACCELERATED CELL DEATH6 alleles, revealing a trade-off between metabolism and defense against biotic stress.
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Publisher
American Society of Plant Biologists (ASPB)
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)