Adsorption of the natural protein surfactant Rsn-2 onto liquid interfaces

Brandani, Giovanni B.; Vance, Steven J.; Schor, Marieke;Cooper, Alan;Kennedy, Malcolm W.; Smith, Brian O.; MacPhee, Cait E.;Cheung, David L. (2017) 'Adsorption of the Natural Protein Biosurfactant Rsn-2 at Liquid Interfaces'. Physical Chemistry Chemical Physics, 19(12), 8584-8594. doi: 10.1039/C6CP07261E

Abstract

To stabilize foams, droplets, and films at liquid interfaces nature has evolved a range of protein biosurfactants. Compared to synthetic surfactants, these combine surface activity with biocompatibility and low solution aggregation. One recently studied example is Rsn-2, a component of the foam nest of the frog {\it Engystomops pustulosus}, which has been predicted to undergo a clamshell-like opening transition at the air-water interface. Using atomistic molecular dynamics simulations and surface tension measurements we study adsorption of Rsn-2 onto air-water and cyclohexane-water interfaces. The protein adsorbs readily at both interfaces, with adsorption mediated by the hydrophobic N-terminus. At the cyclohexane-water interface the clamshell opens, due to the favourable interaction between hydrophobic residues and cyclohexane molecules and penetration of cyclohexane molecules into the protein core. Simulations of deletion mutants showed removal of the N-terminus inhibits interfacial adsorption, consistent with the surface tension measurements. Deletion of the hydrophilic C-terminus also affects adsorption, suggesting that this plays a role in orienting the protein at the interface. The characterisation of the interfacial behaviour gives insight into the factors that control interfacial adsorption of proteins, which may inform new applications of this and similar proteins in areas including drug delivery and food technology and may also be used in the design of synthetic molecules showing similar changes in conformation at interfaces.

Funder

|~|1267880|~|1267881|~|

Publisher

Royal Society of Chemistry

Rights

Attribution-NonCommercial-NoDerivs 3.0 Ireland

cbn

Collections

School of Biological and Chemical Sciences (Scholarly Articles)