Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy

Hillman, Timothy R., Alexandrov, Sergey A., Gutzler, Thomas, & Sampson, David D. (2006). Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy. Optics Express, 14(23), 11088-11102. doi:10.1364/OE.14.011088

Abstract

We utilize Fourier-holographic light scattering angular spectroscopy to record the spatially resolved complex angular scattering spectra of samples over wide fields of view in a single or few image captures. Without resolving individual scatterers, we are able to generate spatially-resolved particle size maps for samples composed of spherical scatterers, by comparing generated spectra with Mie-theory predictions. We present a theoretical discussion of the fundamental principles of our technique and, in addition to the sphere samples, apply it experimentally to a biological sample which comprises red blood cells. Our method could possibly represent an efficient alternative to the time-consuming and laborious conventional procedure in light microscopy of image tiling and inspection, for the characterization of microscopic morphology over wide fields of view.

Publisher

Optica

Rights

CC BY-NC-ND 3.0 IE

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Collections

School of Physics (Scholarly Articles)