Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
Passari, Ajit Kumar Mishra, Vineet Kumar Singh, Garima Singh, Pratibha Kumar, Brijesh Gupta, Vijai Kumar Sharma, Rupak Kumar Saikia, Ratul Donovan, Anthonia O’. Singh, Bhim Pratap
Passari, Ajit Kumar
Mishra, Vineet Kumar
Singh, Garima
Singh, Pratibha
Kumar, Brijesh
Gupta, Vijai Kumar
Sharma, Rupak Kumar
Saikia, Ratul
Donovan, Anthonia O’.
Singh, Bhim Pratap
Publication Date
2017-09-18
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Article
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Passari, Ajit Kumar; Mishra, Vineet Kumar; Singh, Garima; Singh, Pratibha; Kumar, Brijesh; Gupta, Vijai Kumar; Sharma, Rupak Kumar; Saikia, Ratul; Donovan, Anthonia O’. Singh, Bhim Pratap (2017). Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production. Scientific Reports 7 ,
Abstract
Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. In the present investigation, 169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellipticum. Based on their antimicrobial potential, 81 strains were identified by 16rRNA gene analysis, which were taxonomically grouped into 15 genera. All identified strains were screened for their plant growth promoting attributes and, for the presence of modular polyketide synthases (PKSI, PKSII and nonribosomal peptide synthetase (NRPS) gene clusters to correlate the biosynthetic genes with their functional properties. Expression studies and antioxidant potential for four representative strains were evaluated using qRT-PCR and DPPH assay respectively. Additionally, six antibiotics (erythromycin, ketoconazole, fluconazole, chloramphenicol, rifampicin and miconazole) and nine phenolic compounds (catechin, kaempferol, chebulagic acid, chlorogenic acid, Asiatic acid, ferulic acid, arjunic acid, gallic acid and boswellic acid) were detected and quantified using UHPLC-QqQLIT-MS/MS. Furthermore, three strains (BPSAC77, 121 and 101) showed the presence of the anticancerous compound paclitaxel which was reported for the first time from endophytic actinobacteria. This study provides a holistic picture, that endophytic actinobacteria are rich bacterial resource for bioactive natural products, which has a great prospective in agriculture and pharmaceutical industries.
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Springer Nature
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Attribution-NonCommercial-NoDerivs 3.0 Ireland