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In vitro comparative cytotoxicity study of a novel biocidal iodo-thiocyanate complex

Tonoyan, Lilit
; 
Boyd, Aoife
; 
Fleming, Gerard T.A.
; 
Friel, Ruairi
; 
Gately, Carol M.
; 
Mc Cay, Paul H.
; 
O'Flaherty, Vincent
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Identifiers
http://hdl.handle.net/10379/15362
 https://doi.org/10.13025/15551
Publication Date
2018-04-03
Keywords
Iodo-thiocyanate complex (ITC), Hydrogen peroxide, Povidone iodine, Lugol's iodine, HeLa cytotoxicity, In vitro comet assay, CELL CYTO-TOXICITY, COMET ASSAY, HUMAN-BLOOD, DNA-DAMAGE, ANTISEPTICS, LACTOPEROXIDASE, FIBROBLASTS, MECHANISMS, SYSTEM, RANGE
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Article
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Citation
Tonoyan, Lilit, Boyd, Aoife, Fleming, Gerard T. A., Friel, Ruairi, Gately, Carol M., Mc Cay, Paul H., & O'Flaherty, Vincent. (2018). In vitro comparative cytotoxicity study of a novel biocidal iodo-thiocyanate complex. Toxicology in Vitro, 50, 264-273. doi: https://doi.org/10.1016/j.tiv.2018.03.014
Abstract
Novel biocides, which avoid the induction of cross-resistance to antibiotics, are an urgent societal requirement. Here, we compared the cytotoxic and bactericidal effects of a new antimicrobial agent, the iodo-thiocyanate complex (ITC), with those of the common antiseptics, hydrogen peroxide (H2O2), povidone iodine (PVP-I) and Lugol's iodine (Lugol). The antimicrobials were co-incubated for 10 min with HeLa and Escherichia coli. cells in the presence and absence of organic matter (Dulbecco's modified Eagle's medium, supplemented with 10% fetal bovine serum). The cytotoxic concentrations of ITC were equivalent to its bactericidal concentrations (7.8 mu g ml(-1)). By contrast, cytotoxic effects of H2O2, PVP-I and Lugol were apparent at concentrations lower than their bactericidal concentrations (250, 250 and 125 mu g ml(-1), respectively). The cellular effects of ITC were not quenched by organic matter, unlike the other antiseptics. ITC, PVP-I and Lugol had hemolytic effect on horse erythrocytes at high concentrations, while H2O2 showed no hemolysis. ITC, at 30 or 300 mu g ml(-1), did not cause DNA breakage in HeLa cells as assessed by an in vitro comet assay in the absence of S9 metabolic activation, whereas H2O2 caused extensive single-strand DNA breaks. The pronounced antimicrobial potency of ITC and its favorable cytotoxicity profile suggests that ITC should be considered for antiseptic applications.
Funder
Irish Research Council
Enterprise Ireland
School of Natural Sciences, National University of Ireland Galway.
Publisher
Elsevier
Publisher DOI
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Collections
Microbiology (Scholarly Articles)