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Wnt signaling promotes oral but suppresses aboral structures in hydractinia metamorphosis and regeneration

Duffy, D. J.
; 
Plickert, G.
; 
Kuenzel, T.
; 
Tilmann, W.
; 
Frank, U.
Identifiers
http://hdl.handle.net/10379/11281
https://doi.org/10.13025/28427
Repository DOI
10.1242/dev.046631
Publication Date
2010-08-04
Keywords
wnt, tcf, beta-catenin, axis formation, posterior patterning, hydractinia, cnidaria, rnai, metamorphosis, regeneration, organizer, invertebrate, oocytes, gonads, sperm maturation, sperm development, oogonia, cnidarian clytia-hemisphaerica, hydra head regeneration, primary body axis, beta-catenin, planarian regeneration, stem-cells, polarity, echinata, neuropeptides, evolution
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Article
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Citation
Duffy, D. J. Plickert, G.; Kuenzel, T.; Tilmann, W.; Frank, U. (2010). Wnt signaling promotes oral but suppresses aboral structures in hydractinia metamorphosis and regeneration. Development 137 (18), 3057-3066
Abstract
We studied the role of Wnt signaling in axis formation during metamorphosis and regeneration in the cnidarian Hydractinia. Activation of Wnt downstream events during metamorphosis resulted in a complete oralization of the animals and repression of aboral structures (i.e. stolons). The expression of Wnt3, Tcf and Brachyury was upregulated and became ubiquitous. Rescue experiments using Tcf RNAi resulted in normal metamorphosis and quantitatively normal Wnt3 and Brachyury expression. Isolated, decapitated polyps regenerated only heads but no stolons. Activation of Wnt downstream targets in regenerating animals resulted in oralization of the polyps. Knocking down Tcf or Wnt3 by RNAi inhibited head regeneration and resulted in complex phenotypes that included ectopic aboral structures. Multiple heads then grew when the RNAi effect had dissipated. Our results provide functional evidence that Wnt promotes head formation but represses the formation of stolons, whereas downregulation of Wnt promotes stolons and represses head formation.
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Publisher
The Company of Biologists
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)