Publication

Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens

Collins, E.
Glennon, M.
Hanley, S.
Murray, A.-M.
Cormican, M.
Smith, T.
Maher, M.
Repository DOI
Publication Date
2001-11-01
Type
Article
Downloads
Citation
Collins, E. Glennon, M.; Hanley, S.; Murray, A.-M.; Cormican, M.; Smith, T.; Maher, M. (2001). Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens. Journal of Clinical Microbiology 39 (11), 4163-4165
Abstract
DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.
Funder
Publisher
American Society for Microbiology
Publisher DOI
10.1128/jcm.39.11.4163-4165.2001
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland