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Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens

Collins, E.
; 
Glennon, M.
; 
Hanley, S.
; 
Murray, A.-M.
; 
Cormican, M.
; 
Smith, T.
; 
Maher, M.
Identifiers
http://hdl.handle.net/10379/8979
 https://doi.org/10.13025/24494
Publication Date
2001-11-01
Keywords
linked immunosorbent-assay, fecal samples, pcr, jejuni, coli
Type
Article
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Citation
Collins, E. Glennon, M.; Hanley, S.; Murray, A.-M.; Cormican, M.; Smith, T.; Maher, M. (2001). Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens. Journal of Clinical Microbiology 39 (11), 4163-4165
Abstract
DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.
Funder
Publisher
American Society for Microbiology
Publisher DOI
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
cbn
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Externally hosted open access publications with University of Galway authors (1)