Publication

Translocation ofvibrio parahaemolyticusacross anin vitrom cell model

Finn, Rebecca
; 
Ahmad, Tauseef
; 
Coffey, Eleanor T.
; 
Brayden, David J.
; 
Baird, Alan W.
; 
Boyd, Aoife
Identifiers
http://hdl.handle.net/10379/11467
https://doi.org/10.13025/28540
Repository DOI
10.1111/1574-6968.12323
Publication Date
2013-11-21
Keywords
bacterial virulence, mapk, gastroenteritis, microbial pathogenesis, iii secretion systems, epithelial m-cells, escherichia-coli, peyers-patches, transport, mechanism, cholerae, mucosal, barrier, enterotoxicity
Type
Article
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Citation
Finn, Rebecca; Ahmad, Tauseef; Coffey, Eleanor T. Brayden, David J.; Baird, Alan W.; Boyd, Aoife (2013). Translocation ofvibrio parahaemolyticusacross anin vitrom cell model. FEMS Microbiology Letters 350 (1), 65-71
Abstract
Consumption of Vibrio parahaemolyticus via contaminated shellfish results in inflammatory gastroenteritis characterised by severe diarrhoea, nausea and stomach cramps. This study investigated the translocation of V.parahaemolyticus across a Peyer's patch M cell-like Caco-2/Raji B co-culture model system, as M cells represent a primary site of infection for many pathogenic bacteria. Vibrio parahaemolyticus translocated across co-culture monolayers in higher numbers as compared to Caco-2 monolayers. Moreover, the bacteria induced a greater disruption of the transepithelial resistance in M cell-like co-cultures than in Caco-2 monocultures. Virulence factors associated with this pathogen include two type three secretion systems (TTSS-1 and TTSS-2). TTSS-1 had no effect on translocation efficiency, with TTSS-2 exhibiting a modest enhancing effect. ERK activity was required for optimal translocation 1h postinfection, however, neither ERK nor the JNK and p38 MAPK were required at 2h pi. Additionally, TER disruption in response to bacterial infection occurred independently of the TTSS and MAPK activation. It was concluded that V.parahaemolyticus causes TER disruption of M cell-like co-cultures and translocates in high numbers across the M cell-like co-culture monolayer. These data implicate M cells as important sites for V.parahaemolyticus invasion across the intestinal epithelium during infection.
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Publisher
Oxford University Press (OUP)
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)