Publication

Cloning, overexpression in escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from talaromyces emersonii

Waters, D. M.
; 
Murray, P. G.
; 
Miki, Y.
; 
Martinez, A. T.
; 
Tuohy, M. G.
; 
Faulds, C. B.
Identifiers
http://hdl.handle.net/10379/14387
 https://doi.org/10.13025/28553
Publication Date
2012-03-09
Keywords
expression, xylan, gene, purification, alignment, product, DNA
Type
Article
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Citation
Waters, D. M. Murray, P. G.; Miki, Y.; Martinez, A. T.; Tuohy, M. G.; Faulds, C. B. (2012). Cloning, overexpression in escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from talaromyces emersonii. Applied and Environmental Microbiology 78 (10), 3759-3762
Abstract
The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces etnersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.
Funder
Publisher
American Society for Microbiology
Publisher DOI
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)