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Compartmental oxidation of thiol–disulphide redox couples during epidermal growth factor signalling

HALVEY, Patrick J.
; 
WATSON, Walter H.
; 
HANSEN, Jason M.
; 
GO, Young-Mi
; 
SAMALI, Afshin
; 
JONES, Dean P.
Identifiers
http://hdl.handle.net/10379/9266
 https://doi.org/10.13025/24503
Publication Date
2005-03-01
Keywords
epidermal growth factor (egf), glutathione, redox signalling, thioredoxin-1, thioredoxin-2, human mitochondrial thioredoxin, human cell-lines, immunohistochemical localization, hydrogen-peroxide, DNA-binding, stress, glutathione, activation, endocytosis, expression
Type
Article
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Citation
HALVEY, Patrick J. WATSON, Walter H.; HANSEN, Jason M.; GO, Young-Mi; SAMALI, Afshin; JONES, Dean P. (2005). Compartmental oxidation of thiol–disulphide redox couples during epidermal growth factor signalling. Biochemical Journal 386 , 215-219
Abstract
Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.
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Publisher
Portland Press Ltd.
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (1)