Establishment of the european meningococcal strain collection genome library (emsc-gl) for the 2011 to 2012 epidemiological year
Bratcher, Holly B Brehony, Carina Heuberger, Sigrid Pieridou-Bagatzouni, Despo Křížová, Pavla Hoffmann, Steen Toropainen, Maija Taha, Muhamed-Kheir Claus, Heike Tzanakaki, Georgina
Bratcher, Holly B
Brehony, Carina
Heuberger, Sigrid
Pieridou-Bagatzouni, Despo
Křížová, Pavla
Hoffmann, Steen
Toropainen, Maija
Taha, Muhamed-Kheir
Claus, Heike
Tzanakaki, Georgina
Publication Date
2018-05-17
Type
Article
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Bratcher, Holly B; Brehony, Carina; Heuberger, Sigrid; Pieridou-Bagatzouni, Despo; Křížová, Pavla; Hoffmann, Steen; Toropainen, Maija; Taha, Muhamed-Kheir; Claus, Heike; Tzanakaki, Georgina; Erdôsi, Tímea; Galajeva, Jelena; van der Ende, Arie; Skoczyńska, Anna; Pana, Marina; Vaculíková, Alena; Paragi, Metka; Maiden, Martin CJ; Caugant, Dominique A (2018). Establishment of the european meningococcal strain collection genome library (emsc-gl) for the 2011 to 2012 epidemiological year. Eurosurveillance 23 (20), 15-25
Abstract
Invasive meningococcal disease surveillance in Europe combines isolate characterisation and epidemiological data to support public health intervention. A representative European Meningococcal Strain Collection (EMSC) of IMD isolates was obtained, and whole genome sequenced to characterise 799 EMSC isolates from the epidemiological year July 2011-June 2012. To establish a genome library (GL), the isolate information was deposited in the pubMLST.org/neisseria database. Genomes were curated and annotated at 2,429 meningococcal loci, including those defining clonal complex, capsule, antigens, and antimicrobial resistance. Most genomes contained genes encoding B (n=525; 65.7%) or C (n=163; 20.4%) capsules; isolates were genetically highly diverse, with >20 genomic lineages, five of which comprising 60.7% (n=485) of isolates. There were >350 antigenic fine-types: 307 were present once, the most frequent (P1.7-2,4:F5-1) comprised 8% (n=64) of isolates. Each genome was characterised for Bexsero Antigen Sequence Typing (BAST): 25.5% (n=204) of isolates contained alleles encoding the fHbp and/or the PorA VRs vaccine component, but most genomes (n=513; 64.2%) did not contain the NadA component. EMSC-GL will support an integrated surveillance of disease-associated genotypes in Europe, enabling the monitoring of hyperinvasive lineages, outbreak identification, and supporting vaccine programme implementation.
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Publisher
European Centre for Disease Control and Prevention (ECDC)
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Attribution-NonCommercial-NoDerivs 3.0 Ireland