Optimization of sample preparation for the identification and quantification of saxitoxin in proficiency test mussel sample using liquid chromatography-tandem mass spectrometry
Harju, Kirsi Rapinoja, Marja-Leena Avondet, Marc-André Arnold, Werner Schär, Martin Burrell, Stephen Luginbühl, Werner Vanninen, Paula
Harju, Kirsi
Rapinoja, Marja-Leena
Avondet, Marc-André
Arnold, Werner
Schär, Martin
Burrell, Stephen
Luginbühl, Werner
Vanninen, Paula
Publication Date
2015-11-25
Type
Article
Downloads
Citation
Harju, Kirsi; Rapinoja, Marja-Leena; Avondet, Marc-André; Arnold, Werner; Schär, Martin; Burrell, Stephen; Luginbühl, Werner; Vanninen, Paula (2015). Optimization of sample preparation for the identification and quantification of saxitoxin in proficiency test mussel sample using liquid chromatography-tandem mass spectrometry. Toxins 7 (12), 4868-4880
Abstract
Saxitoxin (STX) and some selected paralytic shellfish poisoning (PSP) analogues in mussel samples were identified and quantified with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sample extraction and purification methods of mussel sample were optimized for LC-MS/MS analysis. The developed method was applied to the analysis of the homogenized mussel samples in the proficiency test (PT) within the EQuATox project (Establishment of Quality Assurance for the Detection of Biological Toxins of Potential Bioterrorism Risk). Ten laboratories from eight countries participated in the STX PT. Identification of PSP toxins in naturally contaminated mussel samples was performed by comparison of product ion spectra and retention times with those of reference standards. The quantitative results were obtained with LC-MS/MS by spiking reference standards in toxic mussel extracts. The results were within the z-score of +/- 1 when compared to the results measured with the official AOAC (Association of Official Analytical Chemists) method 2005.06, pre-column oxidation high-performance liquid chromatography with fluorescence detection (HPLC-FLD).
Funder
Publisher
MDPI AG
Publisher DOI
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland