Publication

Concentration of norovirus during wastewater treatment and its impact on oyster contamination

Flannery, J.
; 
Keaveney, S.
; 
Rajko-Nenow, P.
; 
O'Flaherty, V.
; 
Dore, W.
Identifiers
http://hdl.handle.net/10379/11506
 https://doi.org/10.13025/28721
Publication Date
2012-02-24
Keywords
reverse transcription-pcr, norwalk-like viruses, treatment-plant, feline calicivirus, rna bacteriophages, enteric viruses, fecal bacteria, shellfish, removal, inactivation
Type
Article
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Citation
Flannery, J. Keaveney, S.; Rajko-Nenow, P.; O'Flaherty, V.; Dore, W. (2012). Concentration of norovirus during wastewater treatment and its impact on oyster contamination. Applied and Environmental Microbiology 78 (9), 3400-3406
Abstract
The concentrations of Escherichia coli, F-specific RNA bacteriophage (FRNA bacteriophage), and norovirus genogroup I (NoV GI) and norovirus genogroup II (NoV GII) in wastewater were monitored weekly over a 1-year period at a wastewater treatment plant (WWTP) providing secondary wastewater treatment. A total of 49 samples of influent wastewater and wastewater that had been treated by primary and secondary wastewater treatment processes (primary and secondary treated wastewater) were analyzed. Using a real-time reverse transcription-quantitative PCR (RT-qPCR), the mean NoV GI and NoV GII concentrations detected in effluent wastewater were 2.53 and 2.63 log(10) virus genome copies 100 ml(-1), respectively. The mean NoV concentrations in wastewater during the winter period (January to March) (n = 12) were 0.82 (NoV GI) and 1.41 (NoV GII) log units greater than the mean concentrations for the rest of the year (n = 37). The mean reductions of NoV GI and GII during treatment were 0.80 and 0.92 log units, respectively, with no significant difference detected in the extent of NoV reductions due to season. No seasonal trend was detected in the concentrations of E. coli or FRNA bacteriophage in wastewater influent and showed mean reductions of 1.49 and 2.13 log units, respectively. Mean concentrations of 3.56 and 3.72 log(10) virus genome copies 100 ml(-1) for NoV GI and GII, respectively, were detected in oysters sampled adjacent to the WWTP discharge. A strong seasonal trend was observed, and the concentrations of NoV GI and GII detected in oysters were correlated with concentrations detected in the wastewater effluent. No seasonal difference was detected in concentrations of E. coli or FRNA bacteriophage detected in oysters.
Funder
Publisher
American Society for Microbiology
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)