Efficacy of crosslinking on tailoring in vivo biodegradability of fibro-porous decellularized extracellular matrix and restoration of native tissue structure: a quantitative study using stereology methods
Burugapalli, Krishna Chan, Jeffrey C. Y. Kelly, John L. Pandit, Abhay S.
Burugapalli, Krishna
Chan, Jeffrey C. Y.
Kelly, John L.
Pandit, Abhay S.
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Publication Date
2013-09-17
Type
Article
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Citation
Burugapalli, Krishna; Chan, Jeffrey C. Y. Kelly, John L.; Pandit, Abhay S. (2013). Efficacy of crosslinking on tailoring in vivo biodegradability of fibro-porous decellularized extracellular matrix and restoration of native tissue structure: a quantitative study using stereology methods. Macromolecular Bioscience 14 (2), 244-256
Abstract
Cholecyst-derived extracellular matrix (CEM) is a fibro-porous decellularized serosal layer of porcine gall-bladder. CEM loses 90% of its weight at 48h of in vitro collagenase digestion, but takes two months to be completely resorbed in vivo. Carbodiimide (EDC) crosslinking helps tailoring CEM's in vitro collagenase susceptibility. Here, the efficacy of EDC crosslinking on tailoring in vivo biodegradability of CEM is reported. CEM crosslinked with 0.0005 and 0.0033x10(3) M of EDC/mg that lose 80% and 0% of their weight respectively to in vitro collagenase digestion, were present even after 180 days in vivo. Quantitative histopathology using stereology methods confirmed our qualitative observation that even a tiny degree of crosslinking can significantly prolong the rate of in vivo degradation and removal of CEM.
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Publisher
Wiley-Blackwell
Publisher DOI
10.1002/mabi.201300195
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Attribution-NonCommercial-NoDerivs 3.0 Ireland