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Production and characterization of chemically cross-linked collagen scaffolds

Sallent, Ignacio
; 
Capella-Monsonís, Héctor
; 
Zeugolis, Dimitrios I.
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Identifiers
http://hdl.handle.net/10379/15885
 https://doi.org/10.13025/20867
Publication Date
2019-03-07
Keywords
Collagen cross-linking, biophysical characterisation, biomechanical properties, cytotoxicity, macrophage response
Type
Book chapter
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Citation
Sallent I., Capella-Monsonís H., Zeugolis D.I. (2019) Production and Characterization of Chemically Cross-Linked Collagen Scaffolds. In: Sagi I., Afratis N. (eds) Collagen. Methods in Molecular Biology, vol 1944. Humana Press, New York, NY
Abstract
Chemical cross-linking of collagen-based devices is used as a means of increasing the mechanical stability and control the degradation rate upon implantation. Herein, we describe techniques to produce cross-linked with glutaraldehyde (GTA; amine terminal cross-linker), 4-arm polyethylene glycol succinimidyl glutarate (4SP; amine terminal cross-linker), diphenyl phosphoryl azide (DPPA; carboxyl terminal cross-linker) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; carboxyl terminal cross-linker) collagen films. In addition, we provide protocols to characterise the biophysical (swelling), biomechanical (tensile) and biological (metabolic activity, proliferation and viability using human dermal fibroblasts and THP-1 macrophages) properties of the cross-linked collagen scaffolds.
Funder
Science Foundation Ireland
European Regional Development Fund
Horizon 2020
Publisher
Humana Press
Publisher DOI
Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
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CÚRAM Centre for Research in Medical Devices (Book Chapters)